Giardia and Cryptosporidium species and genotypes in coyotes (Canis latrans).

نویسندگان

  • James M Trout
  • Mónica Santín
  • Ronald Fayer
چکیده

Feces and duodenal scrapings were collected from 22 coyotes (Canis latrans) killed in managed hunts in northeastern Pennsylvania. Polymerase chain reaction (PCR) methods were used to detect Giardia and Cryptosporidium spp. PCR-amplified fragments of Giardia and Cryptosporidium spp. SSU-rRNA genes were subjected to DNA sequence analysis for species/genotype determination. Seven coyotes (32%) were positive for G. duodenalis: three assemblage C, three assemblage D, and one assemblage B. Six coyotes (27%) were positive for Cryptosporidium spp. One isolate shared 99.7% homology with C. muris, whereas five others (23%) shared 100% homology with C. canis, coyote genotype. This is the first report on multiple genotypes of Giardia spp. in coyotes and on the prevalence of Cryptosporidium spp. genotypes in coyotes.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Genetic Characterization of Eastern “Coyotes” in Eastern Massachusetts

This study examined the genetic nature and relatedness of Canis latrans (Coyotes) in eastern Massachusetts (i.e., eastern Coyotes). We characterized 67 animals at the mitochondrial DNA control region, and 55 of those at 8 microsatellite loci. Structure analysis and factorial correspondence analysis of the microsatellite genotypes indicated that the eastern Coyotes in Massachusetts clustered wit...

متن کامل

Taxonomic Implications of Morphological and Genetic Differences in Northeastern Coyotes (Coywolves) (Canis latrans × C. lycaon), Western Coyotes (C. latrans), and Eastern Wolves (C. lycaon or C. lupus lycaon)

common in nature, having been documented in amphibians, insects, fish, birds, and especially within closely related plant species (Berger 1973; arnold 1992; Fritz et al. 1994; haddad et al. 1994; Parris et al. 1999; arnold et al. 1999; albert et al. 2006; Schierenbeck and Ellstrand 2009; Meyerson et al. 2010). allendorf et al. (2001) noted that hybridization is more common in fish than in other...

متن کامل

Characterization of Toxoplasma gondii from raccoons (Procyon lotor), coyotes (Canis latrans), and striped skunks (Mephitis mephitis) in Wisconsin identified several atypical genotypes.

During 2005-2006, sera and tissues from raccoons (Procyon lotor), coyotes (Canis latrans), and skunks (Mephitis mephitis) from the state of Wisconsin were tested for Toxoplasma gondii infection. Antibodies to T. gondii were found in 32 of 54 (59.2%) raccoons, 18 of 35 (51.4%) coyotes, and 5 of 7 (71.4%) skunks using the modified agglutination test and a cut-off titer of 1:20. Pooled tissues (br...

متن کامل

Northeastern coyote / coywolf taxonomy and admixture : A meta - analysis

A flurry of recent papers have attempted to taxonomically characterise eastern canids, mainly grey wolves Canis lupus, eastern wolves Canis lycaon or Canis lupus lycaon and northeastern coyotes or coywolves Canis latrans, Canis latrans var. or Canis latrans x C. lycaon, in northeastern North America. In this paper, we performed a meta-analysis on northeastern coyote taxonomy by comparing result...

متن کامل

A survey of the parasites of coyotes (Canis latrans) in New York based on fecal analysis.

Coyotes (Canis latrans) have colonized northeastern North America only within the past 10-80 yr. We examined feces of coyotes in 2000-01 at three sites in New York (USA) to survey parasites in the region. Two cestodes, nine nematodes, five protozoa, one trematode, and two arthropods were identified from 145 coyote fecal samples. Parasite component community diversity was higher (n = 16 species)...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians

دوره 37 2  شماره 

صفحات  -

تاریخ انتشار 2006